Knowledge-enriched characterization of cell populations. Morphological data from photographs can insert to the richness of flow cytometry data. As an example, the figure shows an usually common apoptosis assay working with Annexin V and PI, including mobile imaging to characterize cells in Just about every inhabitants to reveal morphologically distinctive capabilities. These insights could not are already received from multiplex staining on your own.
As samples are acquired on the Attune CytPix Flow Cytometer, the significant-velocity brightfield digicam captures and outlets illustrations or photos of detected occasions in a rate around 6,000 photographs for every 2nd, depending on the flow price and graphic measurement. For larger versatility, Attune Cytometric Application lets you alter the graphic capture frequency as wanted and select a selected gate for graphic capture, if essential.
(B) Uncooked 264.seven macrophages tend to be annotated inaccurately with regard to particle depend due to their complexity and noncircular borders. The number of singlets readily available for analysis boosts after the person trains and employs a far more exact design.
Uncover new insights into your experimental information by visualizing populations applying blended fluorescence and morphology knowledge. Our software interface wizards enable downsampling, concatenation (merged information) and dimensionality tools that are constructed in the software for an intuitive, streamlined knowledge analysis experience.
"An image isn't really quite well worth a thousand dots, but it surely really helps you to see illustrations or photos. It is great to discover pictures that correlate with staining. By way of example, AnnexinV-beneficial cells which are morphologically distinct than healthier cells, or CD14+ monocytes which can be larger sized and much more textured than CD3+/CD4+/CD14- lymphocytes.
Also, it truly is illuminating to check out the amount of doublets slip as a result of standard singlet gates, and terrific to confirm the status of unanticipated double-good activities in my Evaluation."
Optimizing gating system. Even robust guide singlet gating is error-vulnerable and stays a subjective final decision place in Pretty check here much all flow cytometry assays. Imaging can be used to confirm and alter gates to incorporate only single cells of interest.
Reliable impression high-quality even at large throughput Acoustic concentrating in addition to a substantial-speed digicam Merge to image these T cells constantly at very low or substantial flow premiums. Easily change concentrate and digicam settings to satisfy experimental specifications.
The Attune NxT cytometer is definitely the dependable workhorse instrument your lab can rely on for reliable and dependable efficiency. The Attune CytPix product encompasses a high-pace brightfield digital camera and AI-driven picture Evaluation software package, with current upgrades such as machine Studying equipment and an intuitive user interface, enabling Increased knowledge precision, precision, and faster insights into sample biology.
Maybe most of all, these activities consist of cells of Plainly various phenotypes which could lead on to incorrect conclusions about double constructive functions (specifically in exceptional populations).
Seem waves enable specific mobile alignment, causing exceptional details quality and ~10X more quickly sample flow costs.
We Earlier shown the power of imaging CAR-T/Ramos cell interactions. Enable’s have a look at just the population of finest interest, the double optimistic activities, To find out more. We could now use extended morphological parameters (here, circularity and skewness of depth) to even further take a look at the options of such populations and refine gating on these events, rising facts robustness.
Leverage the power of Highly developed technologies and innovation to speed up your discoveries. Trust within the Attune Flow Cytometer family to deliver the reliability, speed, and precision that set your get the job done apart.
Versatile payment plan from Agilent Fiscal Remedies help you have the significant instrumentation you'll need for fast project, or to grow your lab.
Gating on these prolonged parameters permits you to speedily and precisely detect populations of interest and ensure gating tactic with little or no handbook overview. The Attune software package delivers access to state-of-the-artwork equipment Discovering Investigation instruments within an expertly developed user interface, which makes it extremely simple to use.
The Attune Xenith cytometer is really a substantial-parameter spectral analyzer, providing equally spectral unmixing and conventional flow capabilities for detailed and multipurpose cell Assessment.
Agilent Price Promise assures you no less than a decade of instrument use in the day you buy. Or else, we will credit history you While using the residual value of the technique towards an upgraded model.
What seemed to be a homogeneous population is definitely extra various—an Perception that should be thought of when interpreting results.